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Contents: Advantages of Tissue Culture Propagation, Sexual Versus Asexual Propagation). Chapter 1 - Background Chapter 2 - General Media Preparation ( Chemical Components of Media, Preparation of Stock Solutions, Preparation of Media 10, Specific Media Requirements, by Genus) Chapter 3 - Sterile Technique ( Guidelines for Using a Sterile Workstation, Guidelines for Following Good Laboratory Practice, Guidelines for Using Good Sterile Technique). Chapter 4 - Sexual Propagation Methods ( Photographic Life Cycle of Orchids Grown in Tissue Culture, Preparation of Grcen Pod for Seed Sowing, Preparation of Split Pod for Dry-Seed Sowing, Preparation of Spread Flask, Preparation of Replate Flask) Appendix A: Basal Media for Plant Cell and Tissue Culture Appendix B: Orchid Pod-Collection Times Glossary |